Dataset Information

Objective comparison of particle tracking methods.

ABSTRACT: Particle tracking is of key importance for quantitative analysis of intracellular dynamic processes from time-lapse microscopy image data. Because manually detecting and following large numbers of individual particles is not feasible, automated computational methods have been developed for these tasks by many groups. Aiming to perform an objective comparison of methods, we gathered the community and organized an open competition in which participating teams applied their own methods independently to a commonly defined data set including diverse scenarios. Performance was assessed using commonly defined measures. Although no single method performed best across all scenarios, the results revealed clear differences between the various approaches, leading to notable practical conclusions for users and developers.

SUBMITTER: Chenouard N

PROVIDER: S-EPMC4131736 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Objective comparison of particle tracking methods.

Chenouard Nicolas N Smal Ihor I de Chaumont Fabrice F Maška Martin M Sbalzarini Ivo F IF Gong Yuanhao Y Cardinale Janick J Carthel Craig C Coraluppi Stefano S Winter Mark M Cohen Andrew R AR Godinez William J WJ Rohr Karl K Kalaidzidis Yannis Y Liang Liang L Duncan James J Shen Hongying H Xu Yingke Y Magnusson Klas E G KE Jaldén Joakim J Blau Helen M HM Paul-Gilloteaux Perrine P Paul-Gilloteaux Perrine P Roudot Philippe P Kervrann Charles C Waharte François F Tinevez Jean-Yves JY Shorte Spencer L SL Willemse Joost J Celler Katherine K van Wezel Gilles P GP Dan Han-Wei HW Tsai Yuh-Show YS Ortiz de Solórzano Carlos C Olivo-Marin Jean-Christophe JC Meijering Erik E

Nature methods 20140119 3

Particle tracking is of key importance for quantitative analysis of intracellular dynamic processes from time-lapse microscopy image data. Because manually detecting and following large numbers of individual particles is not feasible, automated computational methods have been developed for these tasks by many groups. Aiming to perform an objective comparison of methods, we gathered the community and organized an open competition in which participating teams applied their own methods independentl ...[more]

PMID: 24441936

Similar Datasets

Project description:Sleep quality decreases with aging and thus sleep complaints are prevalent in older adults, particularly for those with cognitive impairment and dementia. For older adults, emerging evidence suggests poor sleep quality increases risk of developing cognitive impairment and dementia. Given the aging population-and the impending economic burden associated with increasing numbers of dementia patients-there is pressing need to improve sleep quality among older adults. As such, research efforts have increased focus on investigating the association between age-related sleep changes and cognitive decline in older adults. Sleep quality is a complex construct to evaluate empirically, and yet the Pittsburg Sleep Quality Index (PSQI) is commonly used in studies as their only measure of sleep quality. Furthermore, the PSQI may not be the best sleep quality measure for older adults, due to its reliance on the cognitive capacity to reflect on the past month. Further study is needed to determine the PSQI's validity among older adults. Thus, the current study examined sleep quality for 78 community dwelling adults 55+ to determine the PSQI's predictive validity for objective sleep quality (as measured by actigraphy). We compared two subjective measures of sleep quality-the PSQI and Consensus Sleep Diary (CSD)-with actigraphy (MotionWatch 8©; camntech). Our results suggest perceived sleep quality is quite different from objective reality, at least for adults 55+. Importantly, we show this difference is unrelated to age, gender, education, or cognitive status (assessed using standard screens). Previous studies have shown the PSQI to be a valuable tool for assessing subjective sleep quality; however, our findings indicate for older adults the PSQI should not be used as a substitute for actigraphy, or vice versa. Hence, we conclude best practice is to include both subjective and objective measures when examining sleep quality in older adults (i.e., the PSQI, CSD, and actigraphy).

Dataset Information

Objective comparison of particle tracking methods.

Publications

Objective comparison of particle tracking methods.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets