Construction of a microsatellites-based linkage map for the white grouper (Epinephelus aeneus).

ABSTRACT: The white grouper (Epinephelus aeneus) is a promising candidate for domestication and aquaculture due to its fast growth, excellent taste, and high market price. A linkage map is an essential framework for mapping quantitative trait loci for economic traits and the study of genome evolution. DNA of a single individual was deep-sequenced, and microsatellite markers were identified in 177 of the largest scaffolds of the sequence assembly. The success rate of developing polymorphic homologous markers was 94.9% compared with 63.1% of heterologous markers from other grouper species. Of the 12 adult mature fish present in the broodstock tank, two males and two females were identified as parents of the assigned offspring by parenthood analysis using 34 heterologous markers. A single full-sib fami

SUBMITTER: Dor L 

PROVIDER: S-EPMC4132176 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature