Proteomic analysis reveals CACN-1 is a component of the spliceosome in Caenorhabditis elegans.

ABSTRACT: Cell migration is essential for embryonic development and tissue formation in all animals. cacn-1 is a conserved gene of unknown molecular function identified in a genome-wide screen for genes that regulate distal tip cell migration in the nematode worm Caenorhabditis elegans. In this study we take a proteomics approach to understand CACN-1 function. To isolate CACN-1-interacting proteins, we used an in vivo tandem-affinity purification strategy. Tandem-affinity purification-tagged CACN-1 complexes were isolated from C. elegans lysate, analyzed by mass spectrometry, and characterized bioinformatically. Results suggest significant interaction of CACN-1 with the C. elegans spliceosome. All of the identified interactors were screened for distal tip cell migration phenotypes using RNAi. Deplet

SUBMITTER: Doherty MF 

PROVIDER: S-EPMC4132184 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature