Expression of mRNA transcripts encoding membrane transporters detected with whole transcriptome sequencing of human brain and liver.

ABSTRACT:

Background

Membrane transporters control the influx and efflux of endogenous and xenobiotic substrates, including nutrients and drugs, across cellular membranes.

Objective

Whole transcriptome sequencing enables simultaneous analysis of overall and allele-specific mRNA expression, and the detection of multiple RNA isoforms.

Methods

Here we characterize variation in RNA transcripts emanating from gene loci encoding transporters based on RNAseq data from 10 human brains (including cocaine overdose and normal brain tissues) and 12 normal livers.

Results

mRNA expression was detected in 65% of transporter genes in either tissue, with many genes generating multiple mRNA transcripts. Single-nucleotide polymorphisms within transporters with previous evidence for pharmac

SUBMITTER: Webb A 

PROVIDER: S-EPMC4132188 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature