PKA-regulated VASP phosphorylation promotes extrusion of transformed cells from the epithelium.

ABSTRACT: At the early stages of carcinogenesis, transformation occurs in single cells within tissues. In an epithelial monolayer, such mutated cells are recognized by their normal neighbors and are often apically extruded. The apical extrusion requires cytoskeletal reorganization and changes in cell shape, but the molecular switches involved in the regulation of these processes are poorly understood. Here, using stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative mass spectrometry, we have identified proteins that are modulated in transformed cells upon their interaction with normal cells. Phosphorylation of VASP at serine 239 is specifically upregulated in Ras(V12)-transformed cells when they are surrounded by normal cells. VASP phosphorylation is required for the cel

SUBMITTER: Anton KA 

PROVIDER: S-EPMC4132389 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature