Compact non-contact total emission detection for in vivo multiphoton excitation microscopy.

ABSTRACT: We describe a compact, non-contact design for a total emission detection (c-TED) system for intra-vital multiphoton imaging. To conform to a standard upright two-photon microscope design, this system uses a parabolic mirror surrounding a standard microscope objective in concert with an optical path that does not interfere with normal microscope operation. The non-contact design of this device allows for maximal light collection without disrupting the physiology of the specimen being examined. Tests were conducted on exposed tissues in live animals to examine the emission collection enhancement of the c-TED device compared to heavily optimized objective-based emission collection. The best light collection enhancement was seen from murine fat (5×-2× gains as a function of depth), whereas mur

SUBMITTER: Combs CA 

PROVIDER: S-EPMC4132828 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature