Enhancing the detection of barcoded reads in high throughput DNA sequencing data by controlling the false discovery rate.

ABSTRACT:

Background

DNA barcodes are short unique sequences used to label DNA or RNA-derived samples in multiplexed deep sequencing experiments. During the demultiplexing step, barcodes must be detected and their position identified. In some cases (e.g., with PacBio SMRT), the position of the barcode and DNA context is not well defined. Many reads start inside the genomic insert so that adjacent primers might be missed. The matter is further complicated by coincidental similarities between barcode sequences and reference DNA. Therefore, a robust strategy is required in order to detect barcoded reads and avoid a large number of false positives or negatives.For mass inference problems such as this one, false discovery rate (FDR) methods are powerful and balanced solutions. Since existing FDR

SUBMITTER: Buschmann T 

PROVIDER: S-EPMC4133078 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature