Low-cost motility tracking system (LOCOMOTIS) for time-lapse microscopy applications and cell visualisation.

ABSTRACT: Direct visualisation of cells for the purpose of studying their motility has typically required expensive microscopy equipment. However, recent advances in digital sensors mean that it is now possible to image cells for a fraction of the price of a standard microscope. Along with low-cost imaging there has also been a large increase in the availability of high quality, open-source analysis programs. In this study we describe the development and performance of an expandable cell motility system employing inexpensive, commercially available digital USB microscopes to image various cell types using time-lapse and perform tracking assays in proof-of-concept experiments. With this system we were able to measure and record three separate assays simultaneously on one personal computer using ident

SUBMITTER: Lynch AE 

PROVIDER: S-EPMC4133191 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature