DNA methylation status is more reliable than gene expression at detecting cancer in prostate biopsy.

ABSTRACT:

Background

We analysed critically the potential usefulness of RNA- and DNA-based biomarkers in supporting conventional histological diagnostic tests for prostate carcinoma (PCa) detection.

Methods

Microarray profiling of gene expression and DNA methylation was performed on 16 benign prostatic hyperplasia (BPH) and 32 cancerous and non-cancerous prostate samples extracted by radical prostatectomy. The predictive value of the selected biomarkers was validated by qPCR-based methods using tissue samples extracted from the 58 prostates and, separately, using 227 prostate core biopsies.

Results

HOXC6, AMACR and PCA3 expression showed the best discrimination between PCa and BPH. All three genes were previously reported as the most promising mRNA-based markers for distinguish

SUBMITTER: Paziewska A 

PROVIDER: S-EPMC4134497 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature