Identification of chemoresistant factors by protein expression analysis with iTRAQ for head and neck carcinoma.

ABSTRACT:

Background

Cisplatin and other anticancer drugs are important in the treatment of head and neck squamous cell carcinoma; however, some tumours develop drug resistance. If chemoresistance could be determined before treatment, unnecessary drug administration would be avoided. Here, we investigated chemoresistance factors by comprehensive analyses at the protein level.

Methods

Four human carcinoma cell lines were used: cisplatin-sensitive UM-SCC-23, UM-SCC-23-CDDPR with acquired cisplatin resistance, naturally cisplatin-resistant UM-SCC-81B, and UM-SCC-23/WR with acquired 5-fluorouracil resistance. Extracted proteins were labelled with iTRAQ and analysed by tandem mass spectrometry to identify resistance. Protein expression was confirmed by western blotting and functional analy

SUBMITTER: Nishimura K 

PROVIDER: S-EPMC4134508 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature