Comprehensive approach to the quantitative analysis of mitochondrial phospholipids by HPLC-MS.

ABSTRACT: A normal-phase HPLC-MS method was established to analyze mitochondrial phospholipids quantitatively as well as qualitatively. An efficient extraction procedure and chromatographic conditions were developed using twelve standardized phospholipids and lysophospholipids. The chromatographic conditions provided physical separation of phospholipids by class, and efficient ionization allowed detection of low abundance phospholipids such as phosphatidylglycerol and monolysocardiolipin. The chromatographic separation of each class of phospholipid permitted qualitative identification of molecular species without interference from other classes. This is advantageous for mitochondrial lipidomics because the composition of mitochondrial phospholipids varies depending on tissue source, pathological con

SUBMITTER: Kim J 

PROVIDER: S-EPMC4135309 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature