Single-molecule detection of a guanine(C8) - thymine(N3) cross-link using ion channel recording.

ABSTRACT: The capability to identify and sequence DNA damage within the context of the genome is an important goal for medical diagnostics. However, currently available methods are not suitable for this purpose. Ion channel nanopore analysis shows promise as a potential single-molecule method to sequence genomic DNA in such a way that also allows detection of base or backbone modifications. Recent studies in human cell lines demonstrated the occurrence of a new DNA cross-link between guanine(C8) and thymine(N3) (5'-G*CT*-3'). The current work presents immobilization and translocation studies of the 5'-G*CT*-3' cross-link in a single-stranded oligodeoxynucleotide using the α-hemolysin (α-HL) ion channel. A 3'-biotinylated DNA strand containing the 5'-G*CT*-3' cross-link was incubated with streptavidi

SUBMITTER: Wolna AH 

PROVIDER: S-EPMC4135377 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature