Remodeling of three-dimensional organization of the nucleus during terminal keratinocyte differentiation in the epidermis.

ABSTRACT: The nucleus of epidermal keratinocytes (KCs) is a complex and highly compartmentalized organelle, whose structure is markedly changed during terminal differentiation and transition of the genome from a transcriptionally active state seen in the basal and spinous epidermal cells to a fully inactive state in the keratinized cells of the cornified layer. Here, using multicolor confocal microscopy, followed by computational image analysis and mathematical modeling, we demonstrate that in normal mouse footpad epidermis, transition of KCs from basal epidermal layer to the granular layer is accompanied by marked differences in nuclear architecture and microenvironment including the following: (i) decrease in the nuclear volume; (ii) decrease in expression of the markers of transcriptionally activ

SUBMITTER: Gdula MR 

PROVIDER: S-EPMC4135477 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature