Leucine-responsive regulatory protein Lrp and PapI homologues influence phase variation of CS31A fimbriae.

ABSTRACT: CS31A, a K88-related surface antigen specified by the clp operon, is a member of the type P family of adhesive factors and plays a key role in the establishment of disease caused by septicemic and enterotoxigenic Escherichia coli strains. Its expression is under the control of methylation-dependent transcriptional regulation, for which the leucine-responsive regulatory protein (Lrp) is essential. CS31A is preferentially in the OFF state and exhibits distinct regulatory features compared to the regulation of other P family members. In the present study, surface plasmon resonance and DNase I protection assays showed that Lrp binds to the distal moiety of the clp regulatory region with low micromolar affinity compared to its binding to the proximal moiety, which exhibits stronger, nanomolar a

SUBMITTER: Graveline R 

PROVIDER: S-EPMC4135631 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature