Intracellular concentrations of 65 species of transcription factors with known regulatory functions in Escherichia coli.

ABSTRACT: The expression pattern of the Escherichia coli genome is controlled in part by regulating the utilization of a limited number of RNA polymerases among a total of its approximately 4,600 genes. The distribution pattern of RNA polymerase changes from modulation of two types of protein-protein interactions: the interaction of core RNA polymerase with seven species of the sigma subunit for differential promoter recognition and the interaction of RNA polymerase holoenzyme with about 300 different species of transcription factors (TFs) with regulatory functions. We have been involved in the systematic search for the target promoters recognized by each sigma factor and each TF using the newly developed Genomic SELEX system. In parallel, we developed the promoter-specific (PS)-TF screening system

SUBMITTER: Ishihama A 

PROVIDER: S-EPMC4135669 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature