Physiological and proteomic analysis of Escherichia coli iron-limited chemostat growth.

ABSTRACT: Iron bioavailability is a major limiter of bacterial growth in mammalian host tissue and thus represents an important area of study. Escherichia coli K-12 metabolism was studied at four levels of iron limitation in chemostats using physiological and proteomic analyses. The data documented an E. coli acclimation gradient where progressively more severe iron scarcity resulted in a larger percentage of substrate carbon being directed into an overflow metabolism accompanied by a decrease in biomass yield on glucose. Acetate was the primary secreted organic by-product for moderate levels of iron limitation, but as stress increased, the metabolism shifted to secrete primarily lactate (∼70% of catabolized glucose carbon). Proteomic analysis reinforced the physiological data and quantified relativ

SUBMITTER: Folsom JP 

PROVIDER: S-EPMC4135680 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature