BEM46 shows eisosomal localization and association with tryptophan-derived auxin pathway in Neurospora crassa.

ABSTRACT: BEM46 proteins are evolutionarily conserved, but their functions remain elusive. We reported previously that the BEM46 protein in Neurospora crassa is targeted to the endoplasmic reticulum (ER) and is essential for ascospore germination. In the present study, we established a bem46 knockout strain of N. crassa. This Δbem46 mutant exhibited a level of ascospore germination lower than that of the wild type but much higher than those of the previously characterized bem46-overexpressing and RNA interference (RNAi) lines. Reinvestigation of the RNAi transformants revealed two types of alternatively spliced bem46 mRNA; expression of either type led to a loss of ascospore germination. Our results indicated that the phenotype was not due to bem46 mRNA downregulation or loss but was caused by the a

SUBMITTER: Kollath-Leiß K 

PROVIDER: S-EPMC4135797 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature