Unknown

Dataset Information

0

Association between pncA gene mutations, pyrazinamidase activity, and pyrazinamide susceptibility testing in Mycobacterium tuberculosis.

ABSTRACT: We determined MICs for, confirmed the presence of pncA mutations in, and performed pyrazinamidase testing on colonies (subclones) obtained from seven isolates that exhibited differential pyrazinamide (PZA) susceptibility. Six of the seven strains were found to exhibit characteristics resulting from the mixture of strains possessing different properties. In addition, our analysis revealed large pncA-spanning deletions (1,565 bp, 4,475 bp, and 6,258 bp) in three strains that showed high PZA resistance.

SUBMITTER: Aono A 

PROVIDER: S-EPMC4136016 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Association between pncA gene mutations, pyrazinamidase activity, and pyrazinamide susceptibility testing in Mycobacterium tuberculosis.

Aono Akio A   Chikamatsu Kinuyo K   Yamada Hiroyuki H   Kato Tomoko T   Mitarai Satoshi S  

Antimicrobial agents and chemotherapy 20140527 8

We determined MICs for, confirmed the presence of pncA mutations in, and performed pyrazinamidase testing on colonies (subclones) obtained from seven isolates that exhibited differential pyrazinamide (PZA) susceptibility. Six of the seven strains were found to exhibit characteristics resulting from the mixture of strains possessing different properties. In addition, our analysis revealed large pncA-spanning deletions (1,565 bp, 4,475 bp, and 6,258 bp) in three strains that showed high PZA resist  ...[more]

Similar Datasets

Unknown Characterization of pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis.
| S-EPMC163747 | biostudies-other
Unknown Correlation between pyrazinamide activity and pncA mutations in Mycobacterium tuberculosis isolates in Taiwan.
| S-EPMC253789 | biostudies-literature
Unknown Characterization of new mutations in pyrazinamide-resistant strains of Mycobacterium tuberculosis and identification of conserved regions important for the catalytic activity of the pyrazinamidase PncA.
| S-EPMC89359 | biostudies-literature
Unknown Mutations associated with pyrazinamide resistance in pncA of Mycobacterium tuberculosis complex organisms.
| S-EPMC163764 | biostudies-other
Unknown Rapid sequencing of the Mycobacterium tuberculosis pncA gene for detection of pyrazinamide susceptibility.
| S-EPMC4313251 | biostudies-literature
Unknown Study of the structure-activity relationships for the pyrazinamidase (PncA) from Mycobacterium tuberculosis.
| S-EPMC1221589 | biostudies-other
Unknown Systematic review of mutations in pyrazinamidase associated with pyrazinamide resistance in Mycobacterium tuberculosis clinical isolates.
| S-EPMC4538510 | biostudies-literature
Unknown High prevalence of phenotypic pyrazinamide resistance and its association with pncA gene mutations in Mycobacterium tuberculosis isolates from Uganda.
| S-EPMC7228079 | biostudies-literature
Unknown Pyrazinamide resistance and mutations in pncA among isolates of Mycobacterium tuberculosis from Khyber Pakhtunkhwa, Pakistan.
| S-EPMC6364397 | biostudies-literature
Unknown Pyrazinamide susceptibility testing of Mycobacterium tuberculosis by high resolution melt analysis.
| S-EPMC3877170 | biostudies-literature