A UV-induced genetic network links the RSC complex to nucleotide excision repair and shows dose-dependent rewiring.

ABSTRACT: Efficient repair of UV-induced DNA damage requires the precise coordination of nucleotide excision repair (NER) with numerous other biological processes. To map this crosstalk, we generated a differential genetic interaction map centered on quantitative growth measurements of >45,000 double mutants before and after different doses of UV radiation. Integration of genetic data with physical interaction networks identified a global map of 89 UV-induced functional interactions among 62 protein complexes, including a number of links between the RSC complex and several NER factors. We show that RSC is recruited to both silenced and transcribed loci following UV damage where it facilitates efficient repair by promoting nucleosome remodeling. Finally, a comparison of the response to high versus lo

SUBMITTER: Srivas R 

PROVIDER: S-EPMC4136507 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature