Grb7 binds to Hax-1 and undergoes an intramolecular domain association that offers a model for Grb7 regulation.

ABSTRACT: Adaptor proteins mediate signal transduction from cell surface receptors to downstream signaling pathways. The Grb7 protein family of adaptor proteins is constituted by Grb7, Grb10, and Grb14. This protein family has been shown to be overexpressed in certain cancers and cancer cell lines. Grb7-mediated cell migration has been shown to proceed through a focal adhesion kinase (FAK)/Grb7 pathway, although the specific participants downstream of Grb7 in cell migration signaling have not been fully determined. In this study, we report that Grb7 interacts with Hax-1, a cytoskeletal-associated protein found overexpressed in metastatic tumors and cancer cell lines. Additionally, in yeast 2-hybrid assays, we show that the interaction is specific to the Grb7-RA and -PH domains. We have also demonstr

SUBMITTER: Siamakpour-Reihani S 

PROVIDER: S-EPMC4136552 | biostudies-literature | 2011 Mar-Apr

REPOSITORIES: biostudies-literature