Assessment of species diversity and distribution of an ancient diatom lineage using a DNA metabarcoding approach.

ABSTRACT:

Background

Continuous efforts to estimate actual diversity and to trace the species distribution and ranges in the natural environments have gone in equal pace with advancements of the technologies in the study of microbial species diversity from microscopic observations to DNA-based barcoding. DNA metabarcoding based on Next Generation Sequencing (NGS) constitutes the latest advancement in these efforts. Here we use NGS data from different sites to investigate the geographic range of six species of the diatom family Leptocylindraceae and to identify possible new taxa within the family.

Methodology/principal findings

We analysed the V4 and V9 regions of the nuclear-encoded SSU rDNA gene region in the NGS database of the European ERA-Biodiversa project BioMarKs, collected in

SUBMITTER: Nanjappa D 

PROVIDER: S-EPMC4136930 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature