Monitoring nanoparticle-mediated cellular hyperthermia with a high-sensitivity biosensor.

ABSTRACT:

Aim

To develop and apply a heat-responsive and secreted reporter assay for comparing cellular response to nanoparticle (NP)- and macroscopic-mediated sublethal hyperthermia.

Materials & methods

Reporter cells were heated by water bath (macroscopic heating) or iron oxide NPs activated by alternating magnetic fields (nanoscopic heating). Cellular responses to these thermal stresses were measured in the conditioned media by secreted luciferase assay.

Results & conclusion

Reporter activity was responsive to macroscopic and nanoparticle heating and activity correlated with measured macroscopic thermal dose. Significant cellular responses were observed with NP heating under doses that were insufficient to measurably change the temperature of the system. Under these conditio

SUBMITTER: Mukherjee A 

PROVIDER: S-EPMC4136989 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature