OX26/CTX-conjugated PEGylated liposome as a dual-targeting gene delivery system for brain glioma.

ABSTRACT:

Background

The successful gene delivery into the brain is a major challenge due to the presence of the blood-brain barrier (BBB). In order to transport plasmid DNA across the BBB and target the brain glioma, the PEGylated liposomes (PLs) modified with OX26 and chlorotoxin (CTX) were developed as a dual-targeting gene delivery system, and the therapeutic efficacy of OX26/CTX-PL/pC27 against glioma was evaluated using in vitro and in vivo experimental models.

Methods

The PEGylated liposome complexes were prepared by the reverse phase evaporation method, and their physicochemical properties were examined. The transfection efficiency, intracellular distribution, in vitro effects of OX26/CTX-PL/pC27 were determined on C6, F98 and HEK293T cell lines. The dual-targeting therapeutic

SUBMITTER: Yue PJ 

PROVIDER: S-EPMC4137094 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature