Strength of selection pressure is an important parameter contributing to the complexity of antibiotic resistance evolution.

ABSTRACT: Revealing the genetic changes responsible for antibiotic resistance can be critical for developing novel antibiotic therapies. However, systematic studies correlating genotype to phenotype in the context of antibiotic resistance have been missing. In order to fill in this gap, we evolved 88 isogenic Escherichia coli populations against 22 antibiotics for 3 weeks. For every drug, two populations were evolved under strong selection and two populations were evolved under mild selection. By quantifying evolved populations' resistances against all 22 drugs, we constructed two separate cross-resistance networks for strongly and mildly selected populations. Subsequently, we sequenced representative colonies isolated from evolved populations for revealing the genetic basis for novel phenotypes. Ba

SUBMITTER: Oz T 

PROVIDER: S-EPMC4137714 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature