Proteomics and metabolomics for in situ monitoring of wound healing.

ABSTRACT: Wound healing of soft tissue and bone defects is a complex process in which cellular differentiation and adaption are regulated by internal and external factors, among them are many different proteins. In contrast to insights into the significance of various single proteins based on model systems, the knowledge about the processes at the actual site of wound healing is still limited. This is caused by a general lack of methods that allow sampling of extracellular factors, metabolites, and proteins in situ. Sampling of wound fluids in combination with proteomics and metabolomics is one of the promising approaches to gain comprehensive and time resolved data on effector molecules. Here, we describe an approach to sample metabolites by microdialysis and to extract proteins simultaneously by a

SUBMITTER: Kalkhof S 

PROVIDER: S-EPMC4137721 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature