Multi-parametric flow cytometric and genetic investigation of the peripheral B cell compartment in human type 1 diabetes.

ABSTRACT: The appearance of circulating islet-specific autoantibodies before disease diagnosis is a hallmark of human type 1 diabetes (T1D), and suggests a role for B cells in the pathogenesis of the disease. Alterations in the peripheral B cell compartment have been reported in T1D patients; however, to date, such studies have produced conflicting results and have been limited by sample size. In this study, we have performed a detailed characterization of the B cell compartment in T1D patients (n = 45) and healthy controls (n = 46), and assessed the secretion of the anti-inflammatory cytokine interleukin (IL)-10 in purified B cells from the same donors. Overall, we found no evidence for a profound alteration of the B cell compartment or in the production of IL-10 in peripheral blood of T1D patients

SUBMITTER: Thompson WS 

PROVIDER: S-EPMC4137841 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature