Metabolite profiling of wheat (Triticum aestivum L.) phloem exudate.

ABSTRACT:

Background

Biofortification of staple crops with essential micronutrients relies on the efficient, long distance transport of nutrients to the developing seed. The main route of this transport in common wheat (Triticum aestivum) is via the phloem, but due to the reactive nature of some essential micronutrients (specifically Fe and Zn), they need to form ligands with metabolites for transport within the phloem. Current methods available in collecting phloem exudate allows for small volumes (μL or nL) to be collected which limits the breadth of metabolite analysis. We present a technical advance in the measurement of 79 metabolites in as little as 19.5 nL of phloem exudate. This was achieved by using mass spectrometry based, metabolomic techniques.

Results

Using gas chromatogr

SUBMITTER: Palmer LJ 

PROVIDER: S-EPMC4138413 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature