Integrin-associated complexes form hierarchically with variable stoichiometry in nascent adhesions.

ABSTRACT:

Background

A complex network of putative molecular interactions underlies the architecture and function of cell-matrix adhesions. Most of these interactions are implicated from coimmunoprecipitation studies using expressed components, but few have been demonstrated or characterized functionally in living cells.

Results

We introduce fluorescence fluctuation methods to determine, at high spatial and temporal resolution, "when" and "where" molecular complexes form and their stoichiometry in nascent adhesions (NAs). We focus on integrin-associated molecules implicated in integrin activation and in the integrin-actin linkage in NAs and show that these molecules form integrin-containing complexes hierarchically within the adhesion itself. Integrin and kindlin reside in a molecular

SUBMITTER: Bachir AI 

PROVIDER: S-EPMC4138543 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature