The histone lysine demethylase Kdm6b is required for activity-dependent preconditioning of hippocampal neuronal survival.

ABSTRACT: Enzymes that regulate histone lysine methylation play important roles in neuronal differentiation, but little is known about their contributions to activity-regulated gene transcription in differentiated neurons. We characterized activity-regulated expression of lysine demethylases and lysine methyltransferases in the hippocampus of adult male mice following pilocarpine-induced seizure. Pilocarpine drove a 20-fold increase in mRNA encoding the histone H3 lysine 27-specific demethylase Kdm6b selectively in granule neurons of the dentate gyrus, and this induction was recapitulated in cultured hippocampal neurons by bicuculline and 4-aminopyridine (Bic + 4AP) stimulation of synaptic activity. Because activity-regulated gene expression is highly correlated with neuronal survival, we tested the

SUBMITTER: Wijayatunge R 

PROVIDER: S-EPMC4138546 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature