Cloning, Expression, and Purification of Recombinant Lysostaphin From Staphylococcus simulans.

ABSTRACT:

Background

Staphylococcus aureus is one of the most common causes of nosocomial infections and its resistance to antibiotics is a global concern. Lysostaphin is an antimicrobial agent belonging to a major class of antimicrobial peptides and proteins known as the bacteriocins. It exhibits a high degree of anti-staphylococcal bacteriolytic activity.

Objectives

In this study, high level of recombinant mature lysostaphin in Escherichia coli was produced by using pET32a expression vector.

Materials and methods

The S. simulans gene encoding lysostaphin was extracted, amplified by polymerase chain reaction (PCR), and sub-cloned in prokaryotic expression vector pET32a. E. coli BL21 (DE3) plysS were transformed with pET32a-lys and gene expression was induced by IPTG. The expre

SUBMITTER: Farhangnia L 

PROVIDER: S-EPMC4138633 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature