Andrographolide induces apoptosis of C6 glioma cells via the ERK-p53-caspase 7-PARP pathway.

ABSTRACT:

Background

Glioma is the most malignant tumor of the central nervous system. Efforts on the development of new chemotherapy are mandatory. Andrographolide (AND), a diterpenoid lactone isolated from the Andrographis paniculata, has been shown to have antitumor activities in several types of cancer cells. Whether AND can exert its antitumor activity in glioblastoma cells remains unknown. This study examined the anticancer effects of AND, both in vitro and in vivo.

Methods

Cell apoptosis was assayed by flow cytometry and nuclear staining. The signaling pathway for AND was determined by western blotting. The effects of AND on tumor growth was evaluated in a mouse model.

Results and conclusion

In vitro, with application of specific inhibitors and siRNA, AND-induced apoptos

SUBMITTER: Yang SH 

PROVIDER: S-EPMC4139087 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature