A robust and adaptable high throughput screening method to study host-microbiota interactions in the human intestine.

ABSTRACT: The intestinal microbiota has many beneficial roles for its host. However, the precise mechanisms developed by the microbiota to influence the host intestinal cell responses are only partially known. The complexity of the ecosystem and our inability to culture most of these micro-organisms have led to the development of molecular approaches such as functional metagenomics, i.e. the heterologous expression of a metagenome in order to identify functions. This elegant strategy coupled to high throughput screening allowed to identify novel enzymes from different ecosystems where culture methods have not yet been adapted to isolate the candidate microorganisms. We have proposed to use this functional metagenomic approach in order to model the microbiota's interaction with the host by combining

SUBMITTER: de Wouters T 

PROVIDER: S-EPMC4139392 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature