Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation.

ABSTRACT: MicroRNAs are short non-coding RNAs that play an important role in the regulation of gene expression. Hence, microRNAs are considered as potential targets for engineering of Chinese hamster ovary (CHO) cells to improve recombinant protein production. Here, we analyzed and compared the microRNA expression patterns of high, low, and non-producing recombinant CHO cell lines expressing two structurally different model proteins in order to identify microRNAs that are involved in heterologous protein synthesis and secretion and thus might be promising targets for cell engineering to increase productivity. To generate reproducible and comparable data, the cells were cultivated in a bioreactor under steady-state conditions. Global microRNA expression analysis showed that mature microRNAs were pred

SUBMITTER: Maccani A 

PROVIDER: S-EPMC4139590 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature