Differential expression of Homeobox C11 protein in water buffalo Bubalus bubalis and its putative 3D structure.

ABSTRACT:

Background

The Homeobox (Hox) family complex contains 39 genes, clustered into four groups (A-D) all expressing in sequential manner. The HOX proteins are transcriptional factors involved in regulation of pattern formation of the anterio-posterior body axis across the species. Most of the Hox family genes have been studied with respect to their organization and expression during the embryonic stages. However, expression pattern of Homeobox C11 (Hoxc11) gene in the 5' region, particularly in higher mammals remains largely unexplored.

Results

We cloned and expressed Homeobox C11 (Hoxc11) gene from water buffalo Bubalus bubalis. The recombinant HOXC11 protein expressed as inclusion bodies was solubilized in Tris buffer (10 mM, pH-6.5) and purified using Ni-NTA affinity column.

SUBMITTER: Sharma M 

PROVIDER: S-EPMC4139611 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature