Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures.

ABSTRACT: Extracellular vesicles (EV) in breast milk carry immune relevant proteins and could play an important role in the instruction of the neonatal immune system. To further analyze these EV and to elucidate their function it is important that native populations of EV can be recovered from (stored) breast milk samples in a reproducible fashion. However, the impact of isolation and storage procedures on recovery of breast milk EV has remained underexposed. Here, we aimed to define parameters important for EV recovery from fresh and stored breast milk. To compare various protocols across different donors, breast milk was spiked with a well-defined murine EV population. We found that centrifugation of EV down into density gradients largely improved density-based separation and isolation of EV, comp

SUBMITTER: Zonneveld MI 

PROVIDER: S-EPMC4139932 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature