Isolation of Chandipura virus (Vesiculovirus: Rhabdoviridae) from Sergentomyia species of sandflies from Nagpur, Maharashtra, India.

ABSTRACT:

Background & objectives

An outbreak of acute encephalitis syndrome was reported from Vidarbha region of Maharashtra s0 tate, India, during July 2012. Anti-IgM antibodies against Chandipura virus (CHPV) were detected in clinical samples. Sandfly collections were done to determine their role in CHPV transmission.

Methods

Twenty nine pools of Sergentomyia spp. comprising 625 specimens were processed for virus isolation in Vero E6 cell line. Diagnostic RT-PCR targeting N-gene was carried out with the sample that showed cytopathic effects (CPE). The PCR product was sequenced, analysed and the sequences were deposited in Genbank database.

Results

CPE in Vero E6 cell line infected with three pools was detected at 48 h post infection. However, virus could be isolated only fro

SUBMITTER: Sudeep AB 

PROVIDER: S-EPMC4140043 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature