Functional and structural properties of a novel protein and virulence factor (Protein sHIP) in Streptococcus pyogenes.

ABSTRACT: Streptococcus pyogenes is a significant bacterial pathogen in the human population. The importance of virulence factors for the survival and colonization of S. pyogenes is well established, and many of these factors are exposed to the extracellular environment, enabling bacterial interactions with the host. In the present study, we quantitatively analyzed and compared S. pyogenes proteins in the growth medium of a strain that is virulent to mice with a non-virulent strain. Particularly, one of these proteins was present at significantly higher levels in stationary growth medium from the virulent strain. We determined the three-dimensional structure of the protein that showed a unique tetrameric organization composed of four helix-loop-helix motifs. Affinity pull-down mass spectrometry anal

SUBMITTER: Wisniewska M 

PROVIDER: S-EPMC4140258 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature