Comparison of epigenetic mediator expression and function in mouse and human embryonic blastomeres.

ABSTRACT: A map of human embryo development that combines imaging, molecular, genetic and epigenetic data for comparisons to other species and across pathologies would be greatly beneficial for basic science and clinical applications. Here, we compared mRNA and protein expression of key mediators of DNA methylation and histone modifications between mouse and human embryos, embryos from fertile/infertile couples, and following growth factor supplementation. We observed that individual mouse and human embryos are characterized by similarities and distinct differences in DNA methylation and histone modification patterns especially at the single-cell level. In particular, while mouse embryos first exhibited sub-compartmentalization of different histone modifications between blastomeres at the morula sta

SUBMITTER: Chavez SL 

PROVIDER: S-EPMC4140471 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature