The LA loop as an important regulatory element of the HtrA (DegP) protease from Escherichia coli: structural and functional studies.

ABSTRACT: Bacterial HtrAs are serine proteases engaged in extracytoplasmic protein quality control and are required for the virulence of several pathogenic species. The proteolytic activity of HtrA (DegP) from Escherichia coli, a model prokaryotic HtrA, is stimulated by stressful conditions; the regulation of this process is mediated by the LA, LD, L1, L2, and L3 loops. The precise mechanism of action of the LA loop is not known due to a lack of data concerning its three-dimensional structure as well as its mode of interaction with other regulatory elements. To address these issues we generated a theoretical model of the three-dimensional structure of the LA loop as per the resting state of HtrA and subsequently verified its correctness experimentally. We identified intra- and intersubunit contacts

SUBMITTER: Figaj D 

PROVIDER: S-EPMC4140941 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature