Impact of phospholipid transfer protein on nascent high-density lipoprotein formation and remodeling.

ABSTRACT:

Objective

Phospholipid transfer protein (PLTP), which binds phospholipids and facilitates their transfer between lipoproteins in plasma, plays a key role in lipoprotein remodeling, but its influence on nascent high-density lipoprotein (HDL) formation is not known. The effect of PLTP overexpression on apolipoprotein A-I (apoA-I) lipidation by primary mouse hepatocytes was investigated.

Approach and results

Overexpression of PLTP through an adenoviral vector markedly affected the amount and size of lipidated apoA-I species that were produced in hepatocytes in a dose-dependent manner, ultimately generating particles that were <7.1 nm but larger than lipid-free apoA-I. These <7.1-nm small particles generated in the presence of overexpressed PLTP were incorporated into mature HDL

SUBMITTER: Ji A 

PROVIDER: S-EPMC4141034 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature