Database search algorithm for identification of intact cross-links in proteins and peptides using tandem mass spectrometry.

ABSTRACT: A new database search algorithm has been developed for identification of intact cross-links in proteins and peptides from tandem mass spectrometric data. Using this algorithm, intact cross-links can be identified and characterized in proteins and peptides with high confidence. The algorithm was tested using BS(3) (bis[sulfosuccinimidyl] suberate) cross-linked Cytochrome C. Five cross-links were identified and verified for spatial plausibility by comparison with its three-dimensional structure at optimized experimental conditions. The distributions of statistical scores for true and false positives and receiver operating characteristic analysis indicate that the algorithm is capable of discriminating true positive cross-linked peptide-spectrum matches from false ones. It has also been demon

SUBMITTER: Xu H 

PROVIDER: S-EPMC4141472 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature