Transfer, imaging, and analysis plate for facile handling of 384 hanging drop 3D tissue spheroids.

ABSTRACT: Three-dimensional culture systems bridge the experimental gap between in vivo and in vitro physiology. However, nonstandardized formation and limited downstream adaptability of 3D cultures have hindered mainstream adoption of these systems for biological applications, especially for low- and moderate-throughput assays commonly used in biomedical research. Here we build on our recent development of a 384-well hanging drop plate for spheroid culture to design a complementary spheroid transfer and imaging (TRIM) plate. The low-aspect ratio wells of the TRIM plate facilitated high-fidelity, user-independent, contact-based collection of hanging drop spheroids. Using the TRIM plate, we demonstrated several downstream analyses, including bulk tissue collection for flow cytometry, high-resolution

SUBMITTER: Cavnar SP 

PROVIDER: S-EPMC4141680 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature