Modified extracorporeal photopheresis with cells from a healthy donor for acute graft-versus-host disease in a mouse model.

ABSTRACT:

Background

Graft-versus-host disease (GvHD) is a major challenge after hematopoietic stem cell transplantation but treatment options for patients are still limited. In many cases first-line treatment with glucocorticoids is not successful. Among second-line therapies the extracorporeal photopheresis (ECP) is frequently performed, due to induction of selective tolerance instead of general immunosuppression. However, for some patients with severe acute GvHD the leukapheresis step of the ECP procedure is physically exhausting and limits the number of ECP cycles.

Methods

We hypothesized that leukocytes from healthy cell donors could be used as a replacement for ECP leukocytes gained from the GvHD patient. For this purpose we used a well established mouse model of acute GvHD. The

SUBMITTER: Budde H 

PROVIDER: S-EPMC4141828 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature