Fluorescence recovery after photobleaching investigation of protein transport and exchange in chromatographic media.

ABSTRACT: A fully-mechanistic understanding of protein transport and sorption in chromatographic materials has remained elusive despite the application of modern continuum and molecular observation techniques. While measuring overall uptake rates in proteins in chromatographic media is relatively straightforward, quantifying mechanistic contributions is much more challenging. Further, at equilibrium in fully-loaded particles, measuring rates of kinetic exchange and diffusion can be very challenging. As models of multicomponent separations rely on accurate depictions of protein displacement and elution, a straightforward method is desired to measure the mobility of bound protein in chromatographic media. We have adapted fluorescence recovery after photobleaching (FRAP) methods to study transport and

SUBMITTER: Traylor SJ 

PROVIDER: S-EPMC4142043 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature