A dual luciferase system for analysis of post-transcriptional regulation of gene expression in Leishmania.

ABSTRACT: Gene expression in kinetoplastid parasites is regulated via post-transcriptional mechanisms that modulate mRNA turnover, translation rate, and/or post-translational protein stability. To facilitate the analysis of post-transcriptional regulation, a dual luciferase system was developed in which firefly and Renilla luciferase reporters genetically fused to compatible drug resistance genes are integrated in place of one allele of the gene of interest and of an internal control gene, respectively, in a manner that preserves the cognate pre-mRNA processing signals. The sensitivity and reproducibility of the assay coupled with the ability to rapidly assemble reporter integration constructs render the dual luciferase system suitable for analysis of multiple candidates derived from global expressi

SUBMITTER: Soysa R 

PROVIDER: S-EPMC4142068 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature