RAD9 enhances radioresistance of human prostate cancer cells through regulation of ITGB1 protein levels.

ABSTRACT:

Background

Mouse embryonic stem cells null for Rad9 are sensitive to deleterious effects of ionizing radiation exposure. Likewise, integrin β1 is a known radioprotective factor. Previously, we showed that RAD9 downregulation in human prostate cancer cells reduces integrin β1 protein levels and ectopic expression of Mrad9 restores inherent high levels.

Methods

We used RNA interference to knockdown Rad9 expression in PC3 and DU145 prostate cancer cells. These cells were then exposed to ionizing radiation, and integrin β1 protein levels were measured by immunoblotting. Survival of irradiated cells was measured by clonogenicity, cell cycle analysis, PARP-1 cleavage, and trypan blue exclusion.

Results

The function of RAD9 in controlling integrin β1 expression is unique and

SUBMITTER: Broustas CG 

PROVIDER: S-EPMC4142073 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature