Analysis of androgenic steroids in environmental waters by large-volume injection liquid chromatography tandem mass spectrometry.

ABSTRACT: A new method was developed for the analysis of natural and synthetic androgenic steroids and their selected metabolites in aquatic environmental matrixes using direct large-volume injection (LVI) high-performance liquid chromatography (HPLC) tandem mass spectrometry (MS/MS). Method accuracy ranged from 87.6 to 108% for analytes with well-matched internal standards. Precision, quantified by relative standard deviation (RSD), was less than 12%. Detection limits for the method ranged from 1.2 to 360 ng/L. The method was demonstrated on a series of 1 h composite wastewater influent samples collected over a day with the purpose of assessing temporal profiles of androgen loads in wastewater. Testosterone, androstenedione, boldenone, and nandrolone were detected in the sample series at concentrat

SUBMITTER: Backe WJ 

PROVIDER: S-EPMC4142814 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature