The Study of DNA Methylation of bax Gene Promoter in Breast and Colorectal Carcinoma Cell Lines.

ABSTRACT:

Background

The Bcl-2 protein family members have known as essential controllersof mitochondrial pathway of apoptosis. Bax has been implicated as potential tumor suppressor in certain solid tumors such as breast and colorectal carcinoma. DNA methylation of promoter associated CpG islands has known as a common mechanism for gene inactivation in tumor cells.

Methods

The Methylation Specific PCR (MSP) has used to find the methylation profile of the bax gene promoter CpG islands in colorectal and breast cancer cell lines.

Results

We have not detected any kind of "CpG islands hypermethylation" in promoter region of the bax gene in T47D, MCF7 (as ER positive), MDA-MB-231 and MDA-MB-468 (as ER negative) breast carcinoma-derived cell lines and colorectal cancer cell lines H29

SUBMITTER: Alipour M 

PROVIDER: S-EPMC4142912 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature