Genomic characterization of three unique Dehalococcoides that respire on persistent polychlorinated biphenyls.

ABSTRACT: Fastidious anaerobic bacteria play critical roles in environmental bioremediation of halogenated compounds. However, their characterization and application have been largely impeded by difficulties in growing them in pure culture. Thus far, no pure culture has been reported to respire on the notorious polychlorinated biphenyls (PCBs), and functional genes responsible for PCB detoxification remain unknown due to the extremely slow growth of PCB-respiring bacteria. Here we report the successful isolation and characterization of three Dehalococcoides mccartyi strains that respire on commercial PCBs. Using high-throughput metagenomic analysis, combined with traditional culture techniques, tetrachloroethene (PCE) was identified as a feasible alternative to PCBs to isolate PCB-respiring Dehaloco

SUBMITTER: Wang S 

PROVIDER: S-EPMC4142991 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature