Structural investigation of ribosomally synthesized natural products by hypothetical structure enumeration and evaluation using tandem MS.

ABSTRACT: Ribosomally synthesized and posttranslationally modified peptides (RiPPs) are a growing class of natural products that are found in all domains of life. These compounds possess vast structural diversity and have a wide range of biological activities, promising a fertile ground for exploring novel natural products. One challenging aspect of RiPP research is the difficulty of structure determination due to their architectural complexity. We here describe a method for automated structural characterization of RiPPs by tandem mass spectrometry. This method is based on the combined analysis of multiple mass spectra and evaluation of a collection of hypothetical structures predicted based on the biosynthetic gene cluster and molecular weight. We show that this method is effective in structural ch

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC4143002 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature